Genotoxicity of waterpipe smoke in buccal cells and peripheral blood leukocytes as determined by comet assay

Journal Article

Print(0)

Inhalation toxicology

Inhal.Toxicol.

Dec

891

896

LR: 20151119; JID: 8910739; 0 (5-hydroxymethyl-2-furancarboxaldehyde); 0 (Benzaldehydes); 0 (Furans); 0 (Pyrones); 0 (Smoke); 6M3C89ZY6R (Nicotine); YC9ST449YJ (ethyl vanillin); OTO: NOTNLM; 2014/10/30 [aheadofprint]; ppublish

England

1091-7691; 0895-8378

PMID: 25357232

eng

Journal Article; Research Support, Non-U.S. Gov't; IM

10.3109/08958378.2014.970787 [doi]

Unknown(0)

25357232

CONTEXT: Waterpipe smoke causes DNA damage in peripheral blood leukocytes and in buccal cells of smokers. OBJECTIVE: To determine the exposure effect of waterpipe smoke on buccal cells and peripheral blood leukocytes in regard to DNA damage using comet assay. MATERIALS AND METHODS: The waterpipe smoke condensates were analyzed by gas chromatography-mass spectrometry (GC-MS). The study was performed on 20 waterpipe smokers. To perform comet assay on bucaal cells of smokers, 10 microl of cell suspension was mixed with 85 microl of pre-warmed 1% low melting agarose, applied to comet slide and electrophoresed. To analyze the effect of smoke condensate in vitro, 1 ml of peripheral blood was mixed with 10 microl of smoke condensate and subjected for comet assay. RESULTS: The GC-MS analysis revealed the presence of 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4on, nicotine, hydroxymethyl furancarboxaldehyde and 3-ethoxy-4-hydroxybenzaldehyde in the smoke condensates. Waterpipe smoking caused DNA damage in vivo in buccal cells of smokers. The tail moment and tail length in buccal cells of smokers were 186 +/- 26 and 456 +/- 71, respectively, which are higher than control. The jurak and moassel smoke condensates were found to cause DNA damage in peripheral blood leukocytes. The moassel smoke condensate was more damaging. DISCUSSION: There is wide misconception that waterpipe smoking is not as harmful as cigarette smoking. This study demonstrated that waterpipe smoke induced DNA damage in exposed cells. CONCLUSION: Waterpipe smokes cause DNA damage in buccal cells. The smoke condensate of both jurak and moassel caused comet formation suggesting DNA damage in peripheral blood leukocytes.

Al-Amrah,H.J., Aboznada,O.A., Alam,M.Z., ElAssouli,M.Z., Mujallid,M.I., ElAssouli,S.M.

Biological Sciences Department, Faculty of Science, King Abdulaziz University , Jeddah , Saudi Arabia and.

20141030

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=25357232

2014