Journal Article
Print(0)
Journal of water and health
J.Water.Health.
Dec
12
4
763
771
JID: 101185420; 0 (DNA, Bacterial); 0 (Drinking Water); E1UOL152H7 (Iron); ppublish
England
1477-8920; 1477-8920
PMID: 25473986
eng
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; IM
10.2166/wh.2014.203 [doi]
Unknown(0)
25473986
A quantitative polymerase chain reaction assay (115 bp amplicon) specific to Escherichia coli K12 with an ABI(TM) internal control was developed based on sequence data encoding the rfb gene cluster. Assay specificity was evaluated using three E. coli K12 strains (ATCC W3110, MG1655 & DH1), 24 non-K12 E. coli and 23 bacterial genera. The biofilm detection limit was 10(3) colony-forming units (CFU) E. coli K12 mL(-1), but required a modified protocol, which included a bio-blocker Pseudomonas aeruginosa with ethylenediaminetetraacetic acid buffered to pH 5 prior to cell lysis/DNA extraction. The novel protocol yielded the same sensitivity for drinking water biofilms associated with Fe3O4 (magnetite)-coated SiO2 (quartz) grains and biofilm-surface iron corrosion products from a drinking water distribution system. The novel DNA extraction protocol and specific E. coli K12 assay are sensitive and robust enough for detection and quantification within iron drinking water pipe biofilms, and are particularly well suited for studying enteric bacterial interactions within biofilms.
Lu,J., Gerke,T.L., Buse,H.Y., Ashbolt,N.J.
US Environmental Protection Agency ORD, NERL, MCEARD, Cincinnati, OH 45268, USA E-mail: Lu.Jingrang@epa.gov; jingrang_lu@yahoo.com.; ORISE, US Environmental Protection Agency ORD, NERL, MCEARD, Cincinnati, OH 45268, USA and Department of Geology, Universi
http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=25473986
2014