Histoplasma capsulatum in planktonic and biofilm forms: in vitro susceptibility to amphotericin B, itraconazole and farnesol

Journal Article

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Journal of medical microbiology

J.Med.Microbiol.

Apr

Pt 4

394

399

CI: (c) 2015; JID: 0224131; 0 (Antifungal Agents); 304NUG5GF4 (Itraconazole); 4602-84-0 (Farnesol); 7XU7A7DROE (Amphotericin B); 2014/12/05 [received]; 2015/01/18 [accepted]; 2015/02/05 [aheadofprint]; ppublish

England

1473-5644; 0022-2615

PMID: 25657300

eng

Journal Article; Research Support, Non-U.S. Gov't; IM

10.1099/jmm.0.000030 [doi]

Unknown(0)

25657300

It is believed that most microbial infections are caused by pathogens organized in biofilms. Recently, it was shown that the dimorphic fungus Histoplasma capsulatum, estimated to be the most common cause of fungal respiratory diseases, is also able to form biofilm. Although the antifungal therapy commonly used is effective, refractory cases and recurrences have been reported. In the search for new compounds with antimicrobial activity, the sesquiterpene farnesol has gained prominence for its antifungal action. This study aimed to evaluate the in vitro susceptibility of H. capsulatum var. capsulatum to the antifungal agents itraconazole and amphotericin B, and farnesol alone and combined, as well as to determine the in vitro antifungal activity of these compounds against biofilms of this pathogen. The results show that farnesol has antifungal activity against H. capsulatum in the yeast and filamentous phases, with MIC values ranging from 0.0078 to 0.00312 microM. A synergistic effect (fractional inhibitory concentration index

The Authors

Brilhante,R.S., de Lima,R.A., Marques,F.J., Silva,N.F., Caetano,E.P., Castelo-Branco Dde,S., Bandeira Tde,J., Moreira,J.L., Cordeiro Rde,A., Monteiro,A.J., Pires de Camargo,Z., Sidrim,J.J., Rocha,M.F.

Specialized Medical Mycology Center (CEMM), Federal University of Ceara, Fortaleza, Ceara, Brazil Federal University of Ceara, Fortaleza, Ceara, Brazil brilhante@ufc.br.; Specialized Medical Mycology Center (CEMM), Federal University of Ceara, Fortaleza,

20150205

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=25657300

2015