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Veterinary microbiology

Vet.Microbiol.

5-Aug

178

4-Mar

238

245

CI: Copyright (c) 2015; JID: 7705469; 0 (Escherichia coli Proteins); EC 2.1.1.- (Methyltransferases); EC 2.1.1.230 (rRNA (adenosine-O-2'-)methyltransferase); EC 3.5.2.6 (beta-Lactamases); OTO: NOTNLM; 2015/03/04 [received]; 2015/05/08 [revised]; 2015/05/1

Netherlands

1873-2542; 0378-1135

PMID: 26013416

eng

Journal Article; Research Support, Non-U.S. Gov't; IM

10.1016/j.vetmic.2015.05.009 [doi]

Unknown(0)

26013416

In the present study, we determined the molecular epidemiology of extended-spectrum beta-lactamases (ESBLs) in Escherichia coli isolated from diseased farmed pigeons in China. A total of 71 E. coli isolates were collected from three pigeon farms from 2011 to 2012 and screened for the presence of the ESBL genes. The ESBLs producers were further tested for the presence of PMQR-encoding genes as well as the 16S rRNA methylase gene using PCR and DNA sequence analysis. Co-transfer of plasmids encoding for ESBLs, PMQR determinants and/or 16S rRNA methylase gene was performed by conjugation into E. coli. The genetic relatedness and plasmid replicon type were determined. A total of 41 ESBLs producers were identified. Only CTX-M type ESBLs were detected, with the most common CTX-M types being CTX-M-65 (n=17), CTX-M-27 (n=11), CTX-M-55 (n=10). Thirty-eight CTX-M-positive isolates were found to harbor at least one PMQR gene, with aac(6')-Ib-cr (n=32) and oqxAB (n=21) being the most prevalent. The rmtB was the only prevalent 16S rRNA methylase gene detected in 24 (58.1%) CTX-M-positive isolates. Although most of the CTX-M producers had distinct pulsotypes, clonal transmission in the same farm was observed. blaCTX-M genes were carried by IncF alone or in combination with IncK plasmids with three different sizes, including 76.8Kb (n=20), 194Kb (n=5), 104.5Kb (n=2). PFGE profiles of CTX-M-positive E. coli isolates indicated potential horizontal spread of these multidrug resistant strains along with those CTX-M encoding genes. Our findings highlight the importance of pigeons as a reservoir of multiple antimicrobial resistance genes.

Elsevier B.V

Yang,L., Yang,L., Lu,D.H., Zhang,W.H., Ren,S.Q., Liu,Y.H., Zeng,Z.L., Jiang,H.X.

College of Veterinary Medicine, Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University (SCAU), Guangzhou 510642, China.; College of Veterinary Medicine, Guangdong Provincial K

20150518

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=26013416

2015