Journal Article

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Environmental research

Environ.Res.

Apr

146

145

153

CI: Copyright (c) 2015; JID: 0147621; 0 (Aerosols); 0 (Air Pollutants); 0 (Isotopes); 2P299V784P (Lead); OTO: NOTNLM; 2015/09/08 [received]; 2015/12/11 [revised]; 2015/12/15 [accepted]; 2016/01/02 [aheadofprint]; ppublish

United States

1096-0953; 0013-9351

PMID: 26752082

eng

Journal Article; IM

10.1016/j.envres.2015.12.017 [doi]

Unknown(0)

26752082

We report progress in using the isotopic composition and concentration of Pb in the dentine and enamel of deciduous teeth to provide a high resolution time frame of exposure to Pb during fetal development and early childhood. Isotope measurements (total Pb and (208)Pb/(206)Pb, (207)Pb/(206)Pb ratios) were acquired by laser ablation inductively coupled mass spectrometry at contiguous 100 micron intervals across thin sections of the teeth; from the outer enamel surface to the pulp cavity. Teeth samples (n=10) were selected from two cohorts of children, aged 5-8 years, living in NE England. By integrating the isotope data with histological analysis of the teeth, using the daily incremental lines in dentine, we were able to assign true estimated ages to each ablation point (first 2-3 years for molars, first 1-2 years for incisors+pre-natal growth). Significant differences were observed in the isotope composition and concentration of Pb between children, reflecting differences in the timing and sources of exposure during early childhood. Those born in 2000, after the withdrawal of leaded petrol in 1999, have the lowest dentine Pb levels (0.4microgPb/g) with (208)Pb/(206)Pb (mean +/-2sigma: 2.145-2.117) (208)Pb/(206)Pb (mean +/-2sigma: 0.898-0.882) ratios that can be modelled as a binary mix between industrial aerosols and leaded petrol emissions. Short duration, high intensity exposure events (1-2 months) were readily identified, together with evidence that dentine provides a good proxy for childhood changes in the isotope composition of blood Pb. Our pilot study confirms that laser ablation Pb isotope analysis of deciduous teeth, when carried out in conjunction with histological analysis, permits a reconstruction of the timing, duration and source of exposure to Pb during early childhood. With further development, this approach has the potential to study larger cohorts and appraise environments where the levels of exposure to Pb are much higher.

Elsevier Inc

Shepherd,T.J., Dirks,W., Roberts,N.M., Patel,J.G., Hodgson,S., Pless-Mulloli,T., Walton,P., Parrish,R.R.

Centre for Oral Health Research, School of Dental Sciences, Newcastle University, Newcastle upon Tyne, UK; British Geological Survey, Keyworth, Nottingham, UK.; Department of Anthropology, Durham University, Durham, UK.; NERC Isotope Geosciences Laborator

20160102

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=26752082

2016