Journal Article

Print(0)

The Journal of antimicrobial chemotherapy

J.Antimicrob.Chemother.

Mar

61

3

504

508

JID: 7513617; EC 3.5.2.6 (beta-Lactamases); 2008/01/25 [aheadofprint]; ppublish

England

1460-2091; 0305-7453

PMID: 18222958

eng

Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; IM

10.1093/jac/dkm517 [doi]

Unknown(0)

18222958

OBJECTIVES: Escherichia coli producing CTX-M-15 enzyme began to rapidly spread in the UK from around 2003 but other types also occur, notably CTX-M-14. We examined breasts from UK-reared (n = 62) and imported (n = 27) chickens as potential sources of quinolone-resistant E. coli with bla(CTX-M) genes. A further 40 samples for which the country of rearing could not be identified were examined. METHODS: During 2006, 129 fresh and frozen chicken breast fillets were purchased from retail outlets in the West Midlands. These were cultured for E. coli on CLED agar containing 8 mg/L ciprofloxacin and carrying a 10 microg cefpodoxime disc. Resistant isolates were identified and typed by RAPD fingerprinting; bla(CTX-M) was identified by PCR and genotyped by reverse-line hybridization. RESULTS: The country of rearing was identified from the packaging for 89 of 129 purchased samples. Only one of the 62 UK-reared chicken samples carried E. coli producing a CTX-M-1 enzyme, whereas 10 of 27 samples reared overseas had E. coli with CTX-M enzymes. Specifically, 4/10 Brazilian, 3/4 Brazilian/Polish/French, and 2/2 Dutch samples had E. coli with CTX-M-2 enzymes. Six of 40 samples for which the country of rearing was not known had producers of CTX-M enzymes, 5 of them with CTX-M-14. CONCLUSIONS: Quinolone-resistant E. coli with various CTX-M beta-lactamase genes that are common in human infections worldwide were found in imported chicken breasts, indicating a possible source for gut colonization. Samples from Brazil were commonly positive for E. coli with CTX-M-2, the dominant bla(CTX-M) genotype from human infections in South America, which is currently rare in clinical infections in the UK. CTX-M-15, the dominant CTX-M type in human infections in the UK, was not found in chicken isolates, suggesting that the UK-reared chickens are not a reservoir of CTX-M-15.

Animals, Brazil, Chickens, Drug Resistance, Bacterial/physiology, Escherichia coli/enzymology/isolation & purification, Escherichia coli Infections/microbiology/prevention & control, Europe, Food Contamination/analysis/prevention & control, Food Microbiology, Great Britain, Meat/microbiology, beta-Lactamases/biosynthesis/isolation & purification

Warren,R. E., Ensor,V. M., O'Neill,P., Butler,V., Taylor,J., Nye,K., Harvey,M., Livermore,D. M., Woodford,N., Hawkey,P. M.

Microbiology Laboratory, Shrewsbury and Telford Hospital NHS Trust, Shrewsbury SY3 8XQ, UK. roderic.warren@homecall.co.uk

20080125

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=18222958

2008