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Systematic and applied microbiology

Syst.Appl.Microbiol.

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26

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302

311

0723-2020

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Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA was used to characterise and compare bacterial communities associated with scallop larvae (Pecten maximus), in different production units in a shellfish hatchery. Water and larvae samples were collected from three different aquaculture systems; stagnant, flow-through and a flow-through system with seawater treated with ozone. Samples were also collected from different algal cultures, inlet tanks and water pipes leading to the different aquaculture systems. Clear differences were seen between the bacterial community associated with the larvae and in the water from the different aquaculture systems. However, there were high similarities in the community composition between different water samples and between larvae samples collected at different time periods, indicating a high stability in the bacterial communities. Fifty three percent of the sequences from these samples were similar to 16S rRNA gene sequences of members of the γ-subclass of the Proteobacteria. The different algal cultures had different bacterial communities, however 73 percent of the sequences were similar to 16S rRNA gene sequences of members of the α-subclass of the Proteobacteria. Differences in the DGGE profiles were also seen between the samples taken from the inlet tanks and water pipes, indicating a change in the bacterial community composition as the water passed through the pipes. To our knowledge this is the first study investigating bacterial communities associated with Great Scallop larvae in different aquaculture systems including noncultured components.

DNA 16S, ozone, RNA 16S, sea water, aquaculture, article, bacterial flora, bacterial genetics, bacterium culture, bacterium identification, bacterium isolate, denaturing gradient gel electrophoresis, gene sequence, Gram negative bacterium, larva, nonhuman, nucleotide sequence, Pecten maximus, polymerase chain reaction, priority journal, scallop, shellfish, water sampling

Embase; MEDLINE

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Sandaa,R. -A, Magnesen,T., Torkildsen,L., Bergh,Ø.

Sandaa, R.-A., University of Bergen, Department of Microbiology, N-5020 Bergen, Norway

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