Journal Article

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Antimicrobial Agents and Chemotherapy

Antimicrob.Agents Chemother.

Aug

54

8

3505

3508

LR: 20141203; GR: R01 AI073289/AI/NIAID NIH HHS/United States; GR: R01 AI073289-01/AI/NIAID NIH HHS/United States; JID: 0315061; 0 (Antifungal Agents); 0 (Echinocandins); 0 (Fungal Proteins); 0 (Glucans); 0 (Polyenes); 0 (Pyrimidines); 7XU7A7DROE (Amphote

United States

1098-6596; 0066-4804

PMID: 20516280

eng

Journal Article; Research Support, N.I.H., Extramural; IM

10.1128/AAC.00227-10 [doi]

Unknown(0)

20516280

Candida infections frequently involve drug-resistant biofilm growth on device surfaces. Glucan synthase gene FKS1 has been linked to triazole resistance in Candida biofilms. We tested the impact of FKS1 modulation on susceptibility to additional antifungal classes. Reduction of FKS1 expression rendered biofilms more susceptible to amphotericin B, anidulafungin, and flucytosine. Increased resistance to anidulafungin and amphotericin B was observed for biofilms overexpressing FKS1. These findings suggest that Candida biofilm glucan sequestration is a multidrug resistance mechanism.

Amphotericin B/pharmacology, Antifungal Agents/classification/pharmacology, Biofilms/drug effects/growth & development, Candida albicans/drug effects/enzymology/genetics/growth & development, Drug Resistance, Fungal, Echinocandins/pharmacology, Flucytosine/pharmacology, Fungal Proteins/genetics/metabolism, Glucans/metabolism, Glucosyltransferases/genetics/metabolism, Humans, Microbial Sensitivity Tests, Polyenes/pharmacology, Pyrimidines/pharmacology

Nett,J. E., Crawford,K., Marchillo,K., Andes,D. R.

Department of Medicine, University of Wisconsin, 600 Highland Ave., H4/572 Clinical Sciences Center, Madison, WI 53792, USA.

20100601

PMC2916329

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=20516280

2010