Widespread distribution of CTX-M and plasmid-mediated AmpC beta-lactamases in Escherichia coli from Brazilian chicken meat

Journal Article

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Memorias do Instituto Oswaldo Cruz

Mem.Inst.Oswaldo Cruz

Apr

110

249

254

LR: 20150707; JID: 7502619; 0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Escherichia coli Proteins); EC 3.5.2.6 (AmpC beta-lactamases); EC 3.5.2.6 (beta-Lactamases); EC 3.5.2.6 (beta-lactamase CTX-M, E coli); OID: NLM: PMC4489457; 2014/10/20 [rec

Brazil

1678-8060; 0074-0276

PMID: 25946250

eng

Journal Article; Research Support, Non-U.S. Gov't; IM

10.1590/0074-02760140389 [doi]

Unknown(0)

25946250

The dissemination of plasmid-mediated antimicrobial resistance genes may pose a substantial public health risk. In the present work, the occurrences of blaCTX-M and plasmid-mediated ampC and qnr genes were investigated in Escherichia coli from 16 chicken carcasses produced by four commercial brands in Brazil. Of the brands tested, three were exporters, including one of organic chicken. Our study assessed 136 E. coli isolates that were grouped into 77 distinct biotypes defined by their origin, resistance profiling, the presence of beta-lactamase and plasmid-mediated quinolone resistance genes and enterobacterial repetitive intergenic consensus-polimerase chain reaction typing. The blaCTX-M-15, blaCTX-M-2 and blaCTX-M-8 genes were detected in one, 17 and eight different biotypes, respectively (45 isolates). Twenty-one biotypes (46 isolates) harboured blaCMY-2. Additionally, blaCMY-2 was identified in isolates that also carried either blaCTX-M-2 or blaCTX-M-8. The qnrB and/or qnrS genes occurred in isolates carrying each of the four types of beta-lactamase determinants detected and also in oxyimino-cephalosporin-susceptible strains. Plasmid-mediated extended-spectrum beta-lactamase (ESBL) and AmpC determinants were identified in carcasses from the four brands tested. Notably, this is the first description of blaCTX-M-15 genes in meat or food-producing animals from South America. The blaCTX-M-8, blaCTX-M-15 and blaCMY-2 genes were transferable in conjugation experiments. The findings of the present study indicate that plasmid-mediated ESBL and AmpC-encoding genes are widely distributed in Brazilian chicken meat.

Botelho,L.A., Kraychete,G.B., Costa e Silva,J.L., Regis,D.V., Picao,R.C., Moreira,B.M., Bonelli,R.R.

Laboratorio de Investigacao em Microbiologia Medica, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.; Laboratorio de Investigacao em Microbiologia Medica, Instituto de Microbiologia Paulo de Go

PMC4489457

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=25946250

2015