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Systematic and applied microbiology

Syst.Appl.Microbiol.

1994/

17

1

44

50

0723-2020

Unknown(0)

Thermophilic, aerobic heterotrophic bacteria, producing a water soluble dark brown melanin-like pigment, were isolated from a hot water pipeline in a small town in southern Iceland. The bacteria stained Gram-negative, are short rods, 1.5 μm long and 0.5 μm in diameter. The cells are usually single or in pairs. A very dense peptidoglycan layer was seen as well as cytoplasmic membrane and an outer membrane. The bacteria are nonmotile and do not form spores. They could grow at 42-73°C with optimum growth temperature of 65°C and optimum pH at 7.5. The bacteria were oxidase and catalase positive, sensitive to the antibiotics penicillin, erythromycin, tetracyclin, chloramphenicol and streptomycin but resistant to polymyxin B and rifampicin. The GC was about 64.5%. The isolates were compared to other known aerobic, heterotrophic bacteria and turned out to resemble to colourless Thermus strain X-1, which was also found to produce the brown pigment. The DNA:DNA similarity between the new isolates and Thermus X-1 was about 83%, whereas the similarity with Thermus aquaticus was about 46%. The new isolates together with strain X-1 are therefore considered to represent a new species, Thermus scotoductus.

ampicillin, antibiotic agent, carbenicillin, catalase, chloramphenicol, erythromycin, gentamicin, kanamycin, meticillin, oxidoreductase, penicillin G, peptidoglycan, polymyxin B, rifampicin, streptomycin, tap water, tetracycline, antibiotic resistance, article, bacterial cell wall, Iceland, nonhuman, priority journal, thermophilic bacterium, Thermus, water contamination

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Kristjansson,J. K., Hjorleifsdottir,S., Marteinsson,V. T., Alfredsson,G. A.

Kristjansson, J.K., Institute of Biology, University of Iceland, 108 Reykjavik, Iceland

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1994