Journal Article

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BMC microbiology

BMC Microbiol.

25-Jun

13

147

2180-13-147

LR: 20150423; JID: 100966981; 0 (Virulence Factors); EC 3.5.2.6 (beta-Lactamases); OID: NLM: PMC3701463; 2013/03/28 [received]; 2013/05/27 [accepted]; 2013/06/25 [aheadofprint]; epublish

England

1471-2180; 1471-2180

PMID: 23800277

eng

Journal Article; Research Support, Non-U.S. Gov't; IM

10.1186/1471-2180-13-147 [doi]

Unknown(0)

23800277

BACKGROUND: Extended-spectrum beta-lactamases (ESBLs), particularly CTX-M- type ESBLs, are among the most important resistance determinants spreading worldwide in Enterobacteriaceae. The aim of this study was to characterize a collection of 163 ESBL-producing Escherichia coli collected in Tunisia, their ESBL-encoding plasmids and plasmid associated addiction systems. RESULTS: The collection comprised 163 ESBL producers collected from two university hospitals of Sfax between 1989 and 2009. 118 isolates harbored blaCTX-M gene (101 blaCTX-M-15 gene and 17 blaCTX-M-14 gene). 49 isolates carried blaSHV-12 gene, 9 blaSHV-2a gene and only 3 blaTEM-26 gene. 16 isolates produced both CTX-M and SHV-12. The 101 CTX-M-15-producing isolates were significantly associated to phylogroup B2 and exhibiting a high number of virulence factors. 24 (23.7%) of the group B2 isolates belonged to clonal complex ST131. Pulsed-field gel electrophoresis (PFGE) typing revealed a genetic diversity of the isolates. 144 ESBL determinants were transferable mostly by conjugation. The majority of plasmid carrying blaCTX-M-15 genes (72/88) were assigned to various single replicon or multireplicon IncF types and had significantly a higher frequency of addiction systems, notably the VagCD module. CONCLUSION: This study demonstrates that the dissemination of CTX-M-15 producing E. coli in our setting was due to the spread of various IncF-type plasmids harboring multiple addiction systems, into related clones with high frequency of virulence determinants.

Mnif,B., Harhour,H., Jdidi,J., Mahjoubi,F., Genel,N., Arlet,G., Hammami,A.

Laboratoire de Microbiologie, Hopital Habib Bourguiba, Sfax, Tunisie. basma_mnif@yahoo.fr

20130625

PMC3701463

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=23800277

2013