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Japanese Journal of Chemotherapy

Jpn.J.Chemother.

2003/01

51

1

8

12

1340-7007

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We isolated 83 Pseudomonas putida strains in the 5 years from January 1997 through December 2001 at Gunma University Hospital. The sample was free of patient duplication. Among them, 27 isolates were resistant to imipenem (IPM), 22 of which were of urine origin. None was isolated from respiratory specimens. Most IPM-resistant isolates were strains multiply resistant to piperacillin, ceftazidime, amikacin, and norfloxacin. The IMP metallo-β-lactamase gene (blaIMP) was identified by PCR from all 27 IPM-resistant strains, which were derived from different 8 wards. We focused on 13 blaIMP-bearing P. putida strains of a ward, 9 isolated from inpatients and 4 detected from around the water pipe. The long-term residence of blaIMP-bearing P. putida strains, identified as the same strains with pulsed-field gel eletrophoresis (PFGE) patterns and MIC patterns as for 10 drugs, was observed in both inpatients and the ward environment. From 9 of the 13 strains, the blaIMP gene was effectively transferred to a recipient strain of Pseudomonas aeruginosa, conferring resistance to IPM and other β-lactams concomitantly with amikacin resistance; 4 of the 9 strains conferred additional resistance to gentamicin and tobramycin.

amikacin, aztreonam, beta lactam antibiotic, beta lactamase, carbapenem derivative, cefepime, ceftazidime, gentamicin, imipenem, meropenem, metalloprotein, norfloxacin, piperacillin, rifampicin, tobramycin, antibiotic resistance, article, bacterial gene, bacterial genetics, bacterial strain, bacterium detection, bacterium identification, bacterium isolate, bacterium isolation, controlled study, gene identification, gene transfer, hospital patient, human, Japan, minimum inhibitory concentration, multidrug resistance, nonhuman, polymerase chain reaction, Pseudomonas aeruginosa, Pseudomonas putida, pulsed field gel electrophoresis, recipient, respiratory system, strain difference, time, university hospital, urinalysis, ward, water supply

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Embase

Yomoda,S., Takahashi,A., Okubo,T., Murakami,M., Iyobe,S.

Yomoda, S., Department of Laboratory Medicine, Clinical Laboratory Center, Bacteria Gunma Univ. School of Med., Maebashi, Japan

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2003