The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation

Journal Article

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Gut

Jan

LR: 20160713; CI: Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.; GR: 092096/Wellcome Trust/United Kingdom; GR: 098357/

England

1468-3288; 0017-5749

PMID: 24572143

eng

Journal Article; Research Support, Non-U.S. Gov't; AIM; IM

10.1136/gutjnl-2013-306171 [doi]

Unknown(0)

24572143

OBJECTIVE: Knowledge of the cellular mechanisms involved in homeostasis of human squamous oesophagus in the steady state and following chronic injury is limited. We aimed to better understand these mechanisms by using a functional 3D approach. DESIGN: Proliferation, mitosis and the expression of progenitor lineage markers were assessed in normal squamous oesophagus from 10 patients by immunofluorescence on 3D epithelial whole mounts. Cells expressing differential levels of epithelial and progenitor markers were isolated using flow cytometry sorting and characterised by qPCR and IF. Their self-renewing potential was investigated by colony forming cells assays and in vitro organotypic culture models. RESULTS: Proliferation and mitotic activity was highest in the interpapillary basal layer and decreased linearly towards the tip of the papilla (p

Barbera,M., di Pietro,M., Walker,E., Brierley,C., MacRae,S., Simons,B.D., Jones,P.H., Stingl,J., Fitzgerald,R.C.

MRC Cancer Unit, University of Cambridge Cambridge, UK.; MRC Cancer Unit, University of Cambridge Cambridge, UK.; MRC Cancer Unit, University of Cambridge Cambridge, UK.; MRC Cancer Unit, University of Cambridge Cambridge, UK.; MRC Cancer Unit, University

20140226

PMC4283695

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=24572143

2015