Fungal infectivities of implanted catheters due to Candida sp. Biofilms formation and resistance

Journal Article

Print(0)

Journal de mycologie medicale

J.Mycol.Med.

Jun

130

135

CI: Copyright (c) 2015; JID: 9425651; 0 (Antifungal Agents); 7XU7A7DROE (Amphotericin B); 8VZV102JFY (Fluconazole); OTO: NOTNLM; 2014/11/25 [received]; 2015/03/01 [revised]; 2015/03/02 [accepted]; 2015/05/06 [aheadofprint]; ppublish

France

1773-0449; 1156-5233

PMID: 25957711

fre

English Abstract; Journal Article; IM

10.1016/j.mycmed.2015.03.003 [doi]

Unknown(0)

25957711

BACKGROUND: Candidemia are the most common fungal infections in hospitals. However, the catheters are subject to be altered by Candida biofilms which increase the risk of invasive nosocomial infections due to the high resistance to antifungal agents. Therefore, the minimum inhibitory concentrations of planktonic (MIC) and sessile cells (CIMS) were evaluated. METHODS: To review the in vivo biofilms structures of Candida sp. formed on the inner and/or external surfaces of collected catheters, we used scanning electron microscopy (SEM). The level of biofilm resistance was assessed against two conventional antifungal agents: amphotericin B (AmB), which belongs to the class of polyenes, and fluconazole (FLZ) which is an azole. RESULTS: The SEM observation of biofilms of Candida sp. reveals complex structures. Compared to MICs, the calculation of CIMS showed an increase of 32 times with AmB and of 128 times with FLZ. CONCLUSION: Catheters offer an ideal surface to Candida sp. to form biofilms. This complex structure induces the increase of the resistance of sessile cells against two antifungal agents, AmB and FLZ.

Elsevier Masson SAS

Seddiki,S.M., Boucherit-Otmani,Z., Boucherit,K., Kunkel,D.

Infectivites fongiques des catheters implantes dues a Candida sp. Formation des biofilms et resistance

Laboratoire antibiotique antifongique, physico-chimie, synthese et activite biologique, universite de Tlemcen, BP 119, 13000 Imama, Tlemcen, Algerie; University center of Naama, BP 66, 45000 Naama, Algerie. Electronic address: seddiki.med@gmail.com.; Labo

20150506

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=25957711

2015