Journal Article

Print(0)

Genetics and molecular research : GMR

Genet.Mol.Res.

14-Aug

14

3

9555

9561

JID: 101169387; 0 (Biomarkers); 0 (Mutagens); 88847-89-6 (8-oxo-7-hydrodeoxyguanosine); G9481N71RO (Deoxyguanosine); epublish

Brazil

1676-5680; 1676-5680

PMID: 26345888

eng

Journal Article; Research Support, Non-U.S. Gov't; IM

10.4238/2015.August.14.18 [doi]

Unknown(0)

26345888

Waterpipe tobacco smoking is increasing in popularity, particularly among young adults. This popularity is related to the lack knowledge regarding the health effects of waterpipe smoking. In this study, we examined the genotoxicity of waterpipe smoking using an 8-hydroxy deoxyguanosine (8-OHdG) assay. Genotoxicity was evaluated in the saliva, urine, and serum of 66 waterpipe adult smokers and 46 healthy nonsmokers. The level of addiction to waterpipe smoking was evaluated using the Lebanon Waterpipe Dependence Scale-11. Levels of 8-OHdG in the samples were measured using commercially available enzyme-linked immunosorbent assays. Levels of 8-OHdG in the saliva (52,430 +/- 2923 vs 48,430 +/- 4189 pg/mL), urine (2815 +/- 312 vs 2608 +/- 180 pg/mL), and serum (19,720 +/- 202 vs 19,670 +/- 254 pg/mL) were similar between waterpipe smokers and nonsmokers (P > 0.05). In addition, no correlations were found between dependence score and levels of 8-OHdG in all sample types. In conclusion, 8-OHdG is not a good biomarker for genotoxic effect of waterpipe smoking.

Azab,M., Khabour,O.F., Alzoubi,K.H., Mahmoud,S.A., Anabtawi,M., Quttina,M.

Department of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, Zarqa, Jordan azabmohd@yahoo.com.; Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.; Department of Clinical Pharmacy

20150814

http://vp9py7xf3h.search.serialssolutions.com/?charset=utf-8&pmid=26345888

2015